The identification of C1q binding domain in the TsPmy N-terminus in this study, together with the C9 binding domain that has been identified within C-terminal 14 amino acid residues (866Val-879Met) [18], provides the feasible design of a multivalent peptide vaccine for preventing infection of T. spiralis in immunized host through efficiently interrupting worm’s ability to evade complement attack. This evidence concerns the gene C9 and infection.