Given the increasing importance of accurate sero-diagnosis of ZIKV infection and the necessity of monitoring humoral immune responses following vaccination with ZIKV vaccines in preclinical and clinical development, we have produced an Asian-lineage ZIKV Env recombinant protein with an optimized secretory profile in mammalian cells, to facilitate large-scale production. This evidence concerns the gene ERVW-1 and Zika virus infectious disease.