On the other hand, the sensitivity of cholangiocarcinoma cells to cisplatin- and 5-FU-induced apoptosis was increased only when galectin-3 expression was almost completely diminished by siRNA (< 10%), whereas the reduction of galectin-3 expression only to 50% did not induce apoptosis [24]. This evidence concerns the gene LGALS3 and cholangiocarcinoma.