The proportions of Annexin V staining cells in 0, 2.5, 5, and 10 μM of DT-13 groups were 6.15, 6.26, 8.47, and 27.0 in PC3 cells and 1.74, 2.45, 10.8, and 18.2% in DU145 cells, indicating DT-13 induced early-phase apoptosis in both prostate cancer cell lines. This evidence concerns the gene ANXA5 and prostate carcinoma.