Quantitative real-time RT-PCR analyses were performed to study the effects of treatment with different DHAs on the expression levels of IC (i.e., CTLA-4, PD-1 and PD-L1) mRNA molecules, in IC-negative cancer cells, selected among the investigated 14 melanoma and 10 hematological cancer cell lines. Here, CD274 is linked to hematopoietic and lymphoid cell neoplasm.