Finally, we attempted to test the assay in 9 CMML patients; 2 patients were mutated for ASXL1 and 2 for SRSF2 (exon 12 and the c.284C>D hotspot, respectively; Additional file 3: Table S3), two frequently mutated genes in CMML [31–33]. Here, SRSF2 is linked to chronic myelomonocytic leukemia.