The growth curve showed that overexpression of RSK4 can inhibit the growth of colorectal cancer cells in vitro, the inhibition at 48 and 60 h was about 60–70%, while the normal group and blank plasmid group was about 10%; the difference has statistical significance (P < 0.05) and the late suppression effect reached a plateau stage. This evidence concerns the gene RPS6KA6 and colorectal cancer.