In addition, four proteins not previously linked to ALS, were selected for further validation; Methanethiol oxidase (SELENBP1), Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (PIN-1), Calcyclin-binding protein (CACYBP) and Rho-associated protein kinase 2 (ROCK 2), these proteins were found significantly dysregulated in our proteomic study. This evidence concerns the gene CACYBP and amyotrophic lateral sclerosis.