While we detected no significant changes for total Nrf2 and KEAP1 (Fig 6 and S1 Fig), we observed a significant nuclear accumulation of Nrf2 by EGb761 in human hepatoma cell lines (Huh7 and WRL68) as well as in IH (THLE5B) (Fig 7), whereby indicating anti-oxidant properties of EGb761 by activation of Nrf2-mediated oxidative stress response. This evidence concerns the gene KEAP1 and hepatocellular carcinoma.