Western blot analysis of melanoma tissues showed increased HIF-1α, carbonic anhydrase IX (CAIX) and vascular endothelial growth factor A (VEGFA) in melanomas with haplo-deficient Phd2 and even more pronounced increase in homozygous deletion of Phd2 compared with tumor tissues from Tyr::CreER; BrafV600E; Pten−/− mice (Fig. 5b). Here, PTEN is linked to melanoma.