We found that the expression levels of ALP, OPN and RUNX2 were positively correlated with each other and the expression of miR-10b was also positively correlated with these bone formation-related genes (Fig. 1B), however, miR-10b was negatively correlated with adipogenic markers CEBPα, PPARγ and AP2 (Supplementary Fig. 1), implying miR-10b might play an important role in osteoporosis. This evidence concerns the gene SPP1 and osteoporosis.