In conclusion, we demonstrated that miR-133b was a pleiotropic tumor suppressor miRNA and targeting FGFR1 as well as the subsequent Ras/MAPK and PI3K/Akt pathways critically mediated the anti-cancer activities of miR-133b in OS cells, including suppressing cell viability, proliferation, migration/invasion, and EMT, and promoting apoptosis. The gene discussed is AKT1; the disease is cancer.