In contrast, Nlgn2 KO mice injected with IgSF9b shRNA showed a pronounced reduction of anxiety-related behaviors compared to Nlgn2 KO mice injected with control shRNA, as evidenced by a significant increase in both the time and the distance traveled in the center (Fig. 4f–g, red vs. red and blue shaded bars, and Fig. 4h, representative traces). This evidence concerns the gene IGSF9B and Anxiety.