NLGN2 and Anxiety: In contrast, Nlgn2 KO mice injected with IgSF9b shRNA showed a pronounced reduction of anxiety-related behaviors compared to Nlgn2 KO mice injected with control shRNA, as evidenced by a significant increase in both the time and the distance traveled in the center (Fig. 4f–g, red vs. red and blue shaded bars, and Fig. 4h, representative traces).