To further confirm that IgSF9b acts in the CeM to modulate anxiety, and to investigate whether targeting IgSF9b-containing synapses in the adult amygdala may recapitulate the anxiolytic effects in the constitutive global KO, we locally reduced IgSF9b levels by adeno-associated virus (AAV)-mediated expression of IgSF9b shRNA (Fig. 4a–b). This evidence concerns the gene IGSF9B and Anxiety.