TIMP3 and infection: In the dentate gyrus, the highest expression of TIMP-3 (Figure 8b) was visible in the infected animal at the beginning of infection (Figure 8b(A)) and then decreased (Figure 8b(B,C)); in the control group the expression of TIMP-3 was similar during the entire period of experiment (Figure 8b(G–I)), immunosuppression decreased TIMP-3 expression (Figure 8b(D–F,J–L)) in comparison to controls, i.e., immunocompetent Acanthamoeba spp.