In U251-MG cells, a similar pattern was observed with fewer cells staining positive for cleaved caspase-3 (between 0.6–1.43% for ZIKVAF, ZIKVCDN and ZIKVPR), whereas approximately 17.1% of cells were cleaved caspase-3-positive during ZIKVBR infection (Figure S2B). The gene discussed is CASP3; the disease is infection.