PRNP and scrapie: To evaluate the influence that PRNP genotype of the sheep scrapie donor had on the seeded conversion of elk PrP, RT-QuIC reactions containing recombinant elk proteins, 132L (Fig 6A) or 132M (Fig 6B), were seeded with 10−2 dilutions of EIA normalized brain tissues from two ARQ/ARQ sheep (#1 and #2) infected with x124 inoculum, two VRQ/VRQ sheep (#3 and #4) infected with x124 inoculum, two VRQ/VRQ sheep (#5 and #6) infected with No. 13–7 inoculum, and two ARQ/ARQ sheep (#7 and #8) infected with No. 13–7 inoculum.