Investigating ischemic stroke subtypes revealed that the detrimental effect of iron status was driven by cardioembolic stroke (serum iron OR, 1.16; 95% CI, 1.01–1.32; P=0.03; [log-transformed] ferritin OR, 1.46; 95% CI, 1.07–2.00; P=0.02; transferrin saturation OR, 1.13; 95% CI, 1.02–1.25; P=0.02; transferrin OR, 0.82; 95% CI, 0.70–0.96; P=0.01). Here, TF is linked to ischemic stroke.