GAS5 and Miyoshi myopathy: To determine whether the effects of GAS5 expression on cell cycle markers influence the regulation of apoptosis in MM cells, we quantified the proportion of cells undergoing apoptosis in populations of A375, A375-GAS5si, SK-Mel-110, and SK-Mel-110-GAS5over cells using Annexin V-APC and 7-AAD double staining and flow cytometry.