The concentration range of TAK-442 used in the assay was expected to be attained in vivo; specifically, in the previous studies, a doubling of the clotting time parameter prothrombin time was observed in a venous thrombosis model in rats orally administrated with TAK-442, and also TAK-442 showed in vitro the prothrombin time doubled in rat plasma at approximately 0.5 μM (Kawamura et al., 2010; Konishi et al., 2010). Here, F2 is linked to Venous thrombosis.