ZIKV infection of hPSC-HLCs and Huh7 cells was confirmed on 4d pi by immunofluorescence staining for ZIKV NS3 and the liver-enriched transcription factor HNF4α, demonstrating 6.4 ± 1.0% and 7.1 ± 2.0% ZIKV-infected hPSC-HLCs and Huh7 cells, respectively (Fig 1B). The gene discussed is HNF4A; the disease is Zika virus infectious disease.