To explore whether the AKT–MDM2–p53 signalling axis is also a downstream target of FAM3B in ESCC, we used Western blot analysis to show that upregulation of FAM3B increased the expression of p‐AKT and MDM2, and decreased the expression of p53, and that silencing of FAM3B produced the opposite effects. This evidence concerns the gene MDM2 and esophageal squamous cell carcinoma.