Different approaches (WB, IF, and IHC) to identify molecules related to mTOR activity such as mTOR, p-mTOR, and its targets, p-p70S6K, p-S6Rb, and p-4E-BP1 were compared in renal cell carcinoma biopsies using different approaches in order to find the best strategy to identify mTORC1 active tumors. Here, RPS6KB1 is linked to hereditary clear cell renal cell carcinoma.