Third, on a rat stroke model, wistar rats received occlusion of the middle cerebral artery for 90 min, liraglutide or saline was administered intraperitoneally at 1 h after reperfusion, liraglutide-treatment significantly reduced the level of derivatives of reactive oxygen metabolites (d-ROMs), compared with that of control, which demonstrated administration of GLP-1 suppressed glucose-stimulated inducible nitric oxide synthase (iNOS) activity and expression and its stimulation of insulin release in pancreatic islet cells at least partly through PKA signalling [35, 36]. This evidence concerns the gene INS and stroke disorder.