Mean methylation of all four CpGs in the first exon of TLR4 were significantly reduced in obese subjects compared to T2D subjects, while no differences in mean methylation were observed between T2D subjects and lean controls. Reduced methylation of seven CpGs in the TLR2 promoter observed in T2D vs. lean group, while no differences observed between obese group and lean controls. Here, TLR4 is linked to type 2 diabetes mellitus.