To examine whether there were age-related differences in the suppressive activity of separated naive CD45RA+ and CD45RA− memory Tregs, the total CD4+CD127low+/–CD25+ Treg pool of 40 healthy volunteers (mean age 41 ± 17 years) and 37 SLE patients (mean age 49 ± 13 years) was isolated by magnetic-activated cell sorting (MACS) and sorted into both Treg subsets (Fig. 8a). This evidence concerns the gene CD4 and systemic lupus erythematosus.