These results do demonstrate that 4-AAQB effectively inhibits the stem cell-like phenotype and attenuates the self-renewal capacity of GBM cells and is consistent with the significant downregulation in expression or marked decrease in nuclear localization of β-catenin, and the stemness marker, Sox2 and Oct4 demonstrated earlier in Figure 3 and Figure 4. This evidence concerns the gene POU5F1 and glioblastoma.