DND1 and in situ carcinoma: We confirmed that expression levels of these genes were also highly correlated with expression levels of DND1 in the MS-CIS (patients from Fig 1) cohort with the peak expression of DND1 occurring between 4–7 months after the reported initial attack of CNS demyelination (Fig 6B, note that for these studies we compared expression levels of DND1, Y-axis, to these other genes since we did not determine IL33 levels in these samples due to limiting amounts of RNA).