To assess the suppressing effect of VGB4 on PI3K/AKT and MAPK/ERK1/2 signaling pathways, western blot analysis of AKT, p-AKT, ERK1/2 and p-ERK1/2 were performed both in vitro (HUVECs) and in vivo (4T1 tumor tissue sections). The gene discussed is MAPK3; the disease is neoplasm.