CD8A and neoplasm: This feature was validated by flow cytometry using fresh isolated biopsies sampled from multiple loci (Fig. 3b). (2) Immunologically hot tumor regions overall had a higher abundance of immune cell infiltration, including cells executing anti-tumor reactivity (e.g., activated CD8+ T cells, type 1 T helper cells, and activated dendritic cells) and cells delivering pro-tumor suppression (e.g., MDSCs, regulatory T cells, immature dendritic cells, and neutrophils).