SLC6A3 and Chagas disease: Yet, non-VL sera show only marginally positive titers (1:1,600–1:3,200) in DAT, suggesting that there must be high amounts of antibodies in VL-positive sera that react with specific epitopes on the DAT antigen and thus cause agglutination at dilutions above 1:3,200.23 Further evidence against the DAT reaction being a reinvention of the Wasserman test comes from the absence of cross-reaction between VL-positive sera and DAT antigen prepared from Trypanosoma cruzi epimastigotes, which has been used for serodiagnosis of Chagas disease.26