In support of an immune-activating mechanism of M-ILP, we observed that human melanoma cell lines exposed to melphalan under mild hyperthermia, aiming to mimic the clinical M-ILP protocol, showed increased surface expression of the heat shock protein Hsp70 but not the “eat-me” molecule calreticulin, which accords with a previous study (22). The gene discussed is CALR; the disease is melanoma.