To address whether the altered expression of the lineage factors was reflected in the numbers of immune cells within the tumours we quantified the numbers of CD4+, CD8+ and Treg cells in splenic tissue and intestinal tissue using immunohistochemical staining (number of was based on the average number of cells in 5 sequential longitudinal sections taken every 100 μm). The gene discussed is CD8A; the disease is neoplasm.