When analyzing colonic length and bowel morphological parameters, INF39 and anakinra counteracted significantly the colonic shortening and improved both the macroscopic and histological features of colitis, as compared with YVAD, thus suggesting that the direct inhibition of NLRP3 or IL-1β receptor blockade can exert significant beneficial effects on tissue parameters related to inflammation than caspase-1 inhibition. This evidence concerns the gene NLRP3 and colitis.