When MHC was expressed in both differentiated MyoD-transduced fibroblasts of normal human and DMD patient for 11 to 21 days by immunostaining, we found that the myotubes from MyoD-transduced fibroblasts of DMD patient showed a significantly reduction of MHC area compared with those of normal human on day 16 and 21 (Figures 3A,B). Here, MYOD1 is linked to Duchenne muscular dystrophy.