Next, we investigated the number of nuclei per these myotubes and confirmed that it was not much difference in myotubes from between normal human and a DMD patient, suggesting that the reduction of MHC expression in DMD patient derived from MyoD-transduced fibroblasts was due to the impairment of the differentiation capacity (Figures 3E,F). The gene discussed is MYOD1; the disease is Duchenne muscular dystrophy.