When the myotubes from the MyoD-transduced fibroblasts of DMD patient were exposed to prednisolone for 11 to 16 days of our differentiation protocol, we found a significant increase in the area of myotubes from the MyoD-transduced DMD fibroblasts treated with prednisolone (Figures 4A,B). The gene discussed is MYOD1; the disease is Duchenne muscular dystrophy.