The activation of FXR by OCA (10 mg/kg/day, administered by oral gavage for 7 weeks) and of TGR5 by 8-week-long diet intervention containing 30 mg/kg/day of INT-777, improved liver steatosis and insulin resistance in rodent model of NAFLD and obesity (Thomas et al., 2009; Cipriani et al., 2010). This evidence concerns the gene INS and metabolic dysfunction-associated steatotic liver disease.