NPM1 and acute myeloid leukemia: An AML/MDS-targeted gene panel (Supplemental Table S1) was negative for mutations of common hematological malignancy-associated genes including JAK2, CALR, DNMT3A, NPM1, FLT3, etc. G-banded karyotype analysis showed a 46,XY,t(13;17)(q12;q12) balanced translocation in all 20 metaphase cells examined (Fig. 1A).