To decipher crosstalk between PDAC tumour cells and S100A8- and S100A9-expressing monocytes, we examined whether stimulation of Panc-1 pancreatic cancer cells with recombinant S100A8-GST or S100A9-GST fusion proteins altered their cytokine secretion, compared to stimulation with the control protein, GST. The gene discussed is S100A8; the disease is pancreatic neoplasm.