To confirm that the observed biological effects on melanoma CSCs are related to selective SCD1 targeting we silenced SCD1 and, after checking the silencing efficiency (Fig. 6a) we analysed the mRNA expression levels of the stem cell markers nanog, cd133, jarid1b and oct4. Consistently with our hypothesis, a decrease in their levels was observed (Fig. 6b). Here, KDM5B is linked to melanoma.