These sites are located across the whole genome, including RNR2, ND1, ND2, CO1, ATP6/8, TR, ND4L, ND4, ND5, ND6, and CYTB. A number of findings that have focused on either specific regions or the whole mitochondrial methylome has identified regions with different patterns of DNA methylation, as shown in mouse and human tissues (brain, liver, breast, and embryonic stem cells) and associated with disease (aging, colon cancer, 143B, and GBM) [16–18, 37–40]. Here, MT-ND5 is linked to malignant colon neoplasm.