BRAF and diffuse astrocytoma: In total, 94 pediatric gliomas were analyzed for the BRAF V600E mutation and 15 were found to harbor this mutation (Fig. 1 and Additional file 1: Table S1): 9/62 (14,5%) grade I gliomas (6 pilocytic astrocytomas (PA) and 3 gangliogliomas (GG)); 2/11 (18,2%) grade II gliomas (1 diffuse astrocytoma (DA) and 1 pleomorphic xanthoastrocytoma (XP) classified as a grade II lesion); 2/13 (15,4%) grade III gliomas (1 anaplastic astrocytoma (AA) and 1 anaplastic XP); and 2/9 (22,2%) grade IV gliomas (one astroblastoma and one glioblastoma (GBM)).