Two cell human breast cell lines: MCF7 (p53 wild-type, estrogen receptor, ER+) and MDA-MB-231 (p53 mutant, estrogen receptor, ER−) were treated by moderate dosage of the anticancer drug ADR for 5 days to induce senescence, by which time more than 60% of the remaining, adherent breast cancer cells entered into cell senescence. Here, ESR1 is linked to breast cancer.