To perform such experimental infections, a cell line bearing a single FBPase allele (Δfbpase-/+), a FBPase null mutant (Δfbpase), and two distinct constitutive add-back rescue cell lines (Δfbpase/FBPase-1 and 2) were generated in the AnTat1.1E pleomorphic strain [26]. The gene discussed is FBP1; the disease is infection.