Western blotting showed that the reconstitution of AMPKα1 and PTEN decreased the level of mTOR, p-mTOR, S6K1, VEGF, HIF-1α, HK2, GLUT1, LDHA and CCND1 but increased p21 and p27 when compared with NC (Fig 6D). As a result, restoration of AMPKα1 and PTEN reduced glycolysis, angiogenesis and proliferation of NPC cells. Here, HIF1A is linked to nasopharyngeal carcinoma.