Chi squared analysis indicated that overexpression of FAM134B was significantly associated with tumor size (P = 0.025), pathological vascular invasion (P = 0.026), differentiation grade (P = 0.023), cancer recurrence (P = 0.044) and portal vein tumor thrombus (P = 0.036) in HCC (Table 1). The gene discussed is RETREG1; the disease is hepatocellular carcinoma.