Finally, we established a model that lacks both T cells and NK cells by depleting NK cells on BABL/c nude mice, the tumor growth inhibition led by TIGIT knockout was significantly rescued in the absence of T cells and NK cells, without significant difference between the CT26-sgRNA1 groups treated with control or anti-asialo-GM-1 (Figure 4G). The gene discussed is TIGIT; the disease is neoplasm.