Employing therapeutic depletion of regulatory T cells to break tolerance to melanoma differentiation antigens, followed by surgery to curatively excise residual B16 primary tumors, we identified the formation of tumor/self (gp100) Ag-specific CD8 T cells in the skin with a CD44hi CD62Llow CD103+ CD69+ CLA+ TRM phenotype (Figure 2) (111). This evidence concerns the gene CD8A and melanoma.