However, healthy individuals with lower AIM2 total methylation and AIM2 CpG3 methylation level (<methylation median) exhibited 2.29-fold (P = 0.011) and 8.29-fold (P < 0.001) increased risks of developing T2DM compared to those with higher methylation levels (≧ methylation median), respectively. The gene discussed is AIM2; the disease is type 2 diabetes mellitus.