PRKAG2 and cardiomyopathy: Finally, we combined iCMs with tissue-forming fibroblasts and an extracellular matrix slurry to generate a 3D CMT assay that recapitulates native cardiac architecture and mechanics, which has been adapted from prior assays applied to study contractility phenotypes of dilated and PRKAG2 cardiomyopathy iPSC models (Boudou et al., 2012, Hinson et al., 2015, Hinson et al., 2016).