Meanwhile, real-time PCR analysis indicated expression of rDMAP1 Y246F but not rDMAP1 Y246F/rBub3 S211A led to a large increase of BIRC3, BIRC5 and NFKB1 mRNA levels in tumour tissues compared with WT counterparts (Additional file 6: Figure S6B), suggesting that BIRC3, BIRC5, NFKB1, whose function is relevant to inflammation, would be the critical downstream genes responsible for the anti-apoptotic-effects in vivo under the regulation by DMAP1/Bub3 complex. Here, BUB3 is linked to neoplasm.