In our previous studies, administration of the well-known carcinogen diethynitrosamine (DEN)20, a choline-deficient, L-amino acid-defined (CDAA) diet21, or bile duct ligation22 induced dominant liver fibrosis in Cygb knockout (Cygb−/−) mice relative to wild-type mice (WT). The gene discussed is CYGB; the disease is Hepatic fibrosis.