CYGB and Hepatic fibrosis: We previously showed that genetic knockout of Cygb promotes hepatic fibrosis and the development of hepatocellular carcinoma, accompanied by increased markers of oxidative stress under the administration of diethylnitrosamine20 or a choline-deficient L-amino acid defined diet21, and that both primary HSCsCygb-null and siCygb-treated HSCsCygb-WT exhibited increased ROS generation and upregulated expression of collagen alpha1(I), Tim-1, Il-6, and Tnfα21.